1,312 research outputs found

    No Easy Way Out: The Effectiveness of Deplatforming an Extremist Forum to Suppress Hate and Harassment

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    Legislators and policymakers worldwide are debating options for suppressing illegal, harmful and undesirable material online. Drawing on several quantitative datasets, we show that deplatforming an active community to suppress online hate and harassment, even with a substantial collective effort involving several tech firms, can be hard. Our case study is the disruption of the largest and longest-running harassment forum Kiwi Farms in late 2022, which is probably the most extensive industry effort to date. We collected complete snapshots of this site and its primary competitor Lolcow Farm, encompassing over 14.7M posts during their lifespan over the past decade. These data are supplemented with a full scrape of the Telegram channel used to disseminate new updates when the forum was down, tweets made by the online community leading the takedown, and search interest and web traffic to the forum spanning two months before and four months after the event. Despite the active participation of several tech firms over a few months, this campaign failed to shut down the forum and remove its objectionable content. While briefly raising public awareness, it led to rapid platform displacement and traffic fragmentation. Part of the activity decamped to Telegram, while traffic shifted from the primary domain to previously abandoned alternatives. The community leading the campaign lost interest quickly, traffic was directed back to the main domain, users quickly returned, and the forum was back online and became even more connected. The net effect was that forum activity, active users, threads, posts and traffic were all cut by about half. Deplatforming a dispersed community using a series of court orders against individual service providers appears unlikely to be very effective if the censor cannot incapacitate the key maintainers, whether by arresting them, enjoining them or otherwise deterring them

    Appendices: Failures, errors and mistakes: A systematic review of the literature

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    Terms such as failure, mistakes, errors, obstacles, and struggle are used interchangeably, but each carry different connotations and discipline-specific meanings. Reactions to experiencing a failure can range as well, from being seen as having educative value to be debilitating. These reactions are based on criteria like environment, prior experiences and individual characteristics, to name a few. The purpose of this chapter is to synthesize and clarify how these terms are articulated and utilized in research studies and commentaries published between 1970 and 2017. Through a systematic literature review, we will discuss similarities and differences in how researchers defined these terms, as well as how these definitions differ by cultural context, discipline, and age of participants. Next, we briefly highlight how our research findings on failure within making and tinkering contexts contribute to our current thinking on failure, mistakes, and errors. Our research included approximately 500 youths and 150 educators situated in a variety of settings that implement making and tinkering programs and/or activities including an informal educational setting (i.e., museum), a formal educational setting (i.e., public middle school), and a hybrid setting (i.e., science center running after-school programming at local school sites). We conclude with open questions and recommendations for the field to consider when conducting research around failures, errors, and mistakes in educational contexts

    Europe’s Care Regimes and the Role of Migrant Care Workers Within Them

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    This paper is an examination of the recent restructuring and subsequent convergence of European long-term care models. This paper also aims to highlight the increased role of migrant care workers and the need for great social and governmental recognition for all care providers. The provision of long term care is complex, divided between state, market and family providers; the state alone could not and does not act as the sole provider of care (Banks 1998). The extent to which different sectors are relied upon is largely dependent on the ideology of the country's welfare state (Timonen and Doyle 2007)

    An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites

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    Abstract. Background: Obtaining single parasite clones is required for many techniques in malaria research. Cloning by limiting dilution using microscopy-based assessment for parasite growth is an arduous and labor-intensive process. An alternative method for the detection of parasite growth in limiting dilution assays is using a commercial ELISA histidine-rich protein II (HRP2) detection kit. Methods. Detection of parasite growth was undertaken using HRP2 ELISA and compared to thick film microscopy. An HRP2 protein standard was used to determine the detection threshold of the HRP2 ELISA assay, and a HRP2 release model was used to extrapolate the amount of parasite growth required for a positive result. Results: The HRP2 ELISA was more sensitive than microscopy for detecting parasite growth. The minimum level of HRP2 protein detection of the ELISA was 0.11ng/ml. Modeling of HRP2 release determined that 2,116 parasites are required to complete a full erythrocytic cycle to produce sufficient HRP2 to be detected by the ELISA. Under standard culture conditions this number of parasites is likely to be reached between 8 to 14 days of culture. Conclusions: This method provides an accurate and simple way for the detection of parasite growth in limiting dilution assays, reducing time and resources required in traditional methods. Furthermore the method uses spent culture media instead of the parasite-infected red blood cells, enabling culture to continue

    Expression quantitative trait loci are highly sensitive to cellular differentiation state

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    Blood cell development from multipotent hematopoietic stem cells to specialized blood cells is accompanied by drastic changes in gene expression for which the triggers remain mostly unknown. Genetical genomics is an approach linking natural genetic variation to gene expression variation, thereby allowing the identification of genomic loci containing gene expression modulators (eQTLs). In this paper, we used a genetical genomics approach to analyze gene expression across four developmentally close blood cell types collected from a large number of genetically different but related mouse strains. We found that, while a significant number of eQTLs (365) had a consistent “static” regulatory effect on gene expression, an even larger number were found to be very sensitive to cell stage. As many as 1,283 eQTLs exhibited a “dynamic” behavior across cell types. By looking more closely at these dynamic eQTLs, we show that the sensitivity of eQTLs to cell stage is largely associated with gene expression changes in target genes. These results stress the importance of studying gene expression variation in well-defined cell populations. Only such studies will be able to reveal the important differences in gene regulation between different ce

    Multi-parallel qPCR provides increased sensitivity and diagnostic breadth for gastrointestinal parasites of humans: field-based inferences on the impact of mass deworming

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    BACKGROUND: Although chronic morbidity in humans from soil transmitted helminth (STH) infections can be reduced by anthelmintic treatment, inconsistent diagnostic tools make it difficult to reliably measure the impact of deworming programs and often miss light helminth infections. METHODS: Cryopreserved stool samples from 796 people (aged 2-81 years) in four villages in Bungoma County, western Kenya, were assessed using multi-parallel qPCR for 8 parasites and compared to point-of-contact assessments of the same stools by the 2-stool 2-slide Kato-Katz (KK) method. All subjects were treated with albendazole and all Ascaris lumbricoides expelled post-treatment were collected. Three months later, samples from 633 of these people were re-assessed by both qPCR and KK, re-treated with albendazole and the expelled worms collected. RESULTS: Baseline prevalence by qPCR (n = 796) was 17 % for A. lumbricoides, 18 % for Necator americanus, 41 % for Giardia lamblia and 15% for Entamoeba histolytica. The prevalence was <1% for Trichuris trichiura, Ancylostoma duodenale, Strongyloides stercoralis and Cryptosporidium parvum. The sensitivity of qPCR was 98% for A. lumbricoides and N. americanus, whereas KK sensitivity was 70% and 32%, respectively. Furthermore, qPCR detected infections with T. trichiura and S. stercoralis that were missed by KK, and infections with G. lamblia and E. histolytica that cannot be detected by KK. Infection intensities measured by qPCR and by KK were correlated for A. lumbricoides (r = 0.83, p < 0.0001) and N. americanus (r = 0.55, p < 0.0001). The number of A. lumbricoides worms expelled was correlated (p < 0.0001) with both the KK (r = 0.63) and qPCR intensity measurements (r = 0.60). CONCLUSIONS: KK may be an inadequate tool for stool-based surveillance in areas where hookworm or Strongyloides are common or where intensity of helminth infection is low after repeated rounds of chemotherapy. Because deworming programs need to distinguish between populations where parasitic infection is controlled and those where further treatment is required, multi-parallel qPCR (or similar high throughput molecular diagnostics) may provide new and important diagnostic information
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